HBV related ELISA Kits

HBsAg S, Pre-S1, Pre-S2 Qualitative Kits  

Product outline

Owing to deep research experience in HBV envelop, Beacle launches quantitative ELISA kits targeted to varous HBsAg domains. Three types of HBsAg is known; named S-, M- and L-antigen. L-antigen is consisted of S, Pre-S2 and Pre-S1 domains, M-antigen of S and Pre-S2 domains, and S-antigen of S domain alone. These domains play important role in HBV infection. Pre-S1 domain is known to be the key element in recognizing hepatic cells, Pre-S2 and S is important in cellular invasion fo HBV. The serum level of S-antigen (or S-domain) is well studies as a tool to daignose the HBV infection, but almost no study was conducted to examine the serum level of Pre-S1 and Pre-S2. Beacle, by marketing ELISA kit to detect these domains, wishes to contribute studies of HBV infection.
Products listed in this page can be ordered directly to us through oder form, or can be odered through our distributors B-Bridge International (USA, Europe), Takara-Bio, Cosmobio, or Funakoshi.

Products properties

HBs antigen quantitative ELISA kits
Pre-S1 quantitative ELISA kit, Rapid
・Only one product in the world that can detect Pre-S1 domains
・High sensitivity:detection limit is 0.1 nUnit/ml
・Possible to determine Pre-S1 antivity in human serum or plasma
・High specificity: Sandwich system using two types of anti-Pre-S1 antibodies
For detail see leaflet

Pre-S2 quantitative ELISA kit, Rapid
・Only one product in the world that can detect Pre-S2 domains
・High sensitivity:detection limit is 0.1 nUnit/ml
・High specificity:Sandwich system using two types of anti-Pre-S2 antibodies
For detail see leaflet

S-antigen quantitative ELISA kit, Rapid-II
・High sensitivity:detection limit is 0.05 nUnit/ml (1 nUnit is roguhly equal to 0.5 to 1 IU of WHO HBsAg standard
・High specificity:Sandwich system using two types of anti-S antibodies
For detail see leaflet

Anti-HBs antibody quantitative ELISA kits
Anti-Pre-S1 antibody quantitative ELISA kit
・ELISA kit to quantitative determination of anti-Pre-S1 antibody
・Kits are available for antibodies bound to genotype A, B,C and D
・Kits for human or mouse serum, other species may be also available
・The system used 2nd antibody detection of sample antibody bound to immobilized Pre-S1
For detail see leaflet

Anti-Pre-S2 antibody quantitative ELISA kit
・ELISA kit to quantitative determination of anti-Pre-S2 antibody
・Kits are available for antibodies bound to genotype A, B,C and D
・Kits for human or mouse serum, other species may be also available
・The system used 2nd antibody detection of sample antibody bound to immobilized Pre-S2
For detail see leaflet

Anti-S antibody quantitative ELISA kit
・ELISA kit to quantitative determination of anti-S antibody
・Kits for human or mouse serum, other species may be also available
・The system used 2nd antibody detection of sample antibody bound to immobilized S antigen
For detail see leaflet

Kit components
All kits are equipped with all the necessary components
For detail please check the working manual for each product

Product lineup and Price

HBs antigen quantitative ELISA kits

Product #Product nameQuantityPrice
BCL-S1HP-01HB Pre-S1 Antigen Quantitative ELISA Kit,Rapid1 Kit (96 assay)¥85,000
BCL-S1D-01HB Pre-S1 Antigen Quantitative ELISA Kit (Genotype D)1 Kit (96 assay)¥85,000
BCL-S2HP-01HB Pre-S2 Antigen Quantitative ELISA Kit,Rapid1 Kit (96 assay)¥85,000
BCL-SHP-21HB S Antigen Quantitative ELISA Kit,Rapid-Ⅱ1 Kit (96 assay)¥65,000

Anti-HBs antibody quantitative ELISA kits

Product #Product nameQuantityPrice
BCL-AKS-01Anti-HB S antibody Quantitative ELISA Kit
(please specify  animal species to measure)
1 Kit (96 assay)inquiry
BCL-AKP1-01Anti-HB Pre-S1 antibody Quantitative ELISA Kit
(please specify  animal species to measure)
1 Kit (96 assay)inquiry
BCL-AKP2-01Anti-HB Pre-S2 antibody Quantitative ELISA Kit
(please specify  animal species to measure)
1 Kit (96 assay)inquiry

Antigens and antibodies for ELISA construction, refer to antigen and antibody page.

Q&A

For HB Pre-S1 Antigen Quantitative ELISA Kit

Q: How long the kit can preserve?

All the component of the kit is stable for at least 6 months at 4℃.

Q: How long does it take for an assay

The approximate time required for an assay is shown below.
1) Antigen ELISA kits: Reaction with capture IgG and detection IgG: 60 min→5) Chromogenic reacton: 30 min
2) Antibody ELISA kits: Reaction with capture antigen blocking, and detection IgG: 2 hrs→5) Chromogenic reacton: 30 min

Q: What is the definition of PreS-1 activity?

There is no common and standard definition of Pre-S1 antigen activity. In the kit the activity is expressed by our unit system, where 1 nUnit equal to the Pre-S1 activity expressed by 1ng of our standard antigen. For S antibody, our kit uses mouse IgG for standard. The binding analysis of the two standard revealed that 10mIU/mL of WHO standard serum is roughly equivalent to about 1nUnit/mL. Please remember that the estimation is not accurate because the binding property of the standard antibody to S antigen is different from that of WHO standard serum in both Bmax and Kd.  

Q: What is the standrd antigen or standard antibody?

The standard antigen are the recombinant HBsAgs produced from yeast. These antigens gives reproducible antigen activity by antigen ELISA. For antibody ELISA mouse monoclonal antibody is provided for standard antibody in kit for mouse

Q: How can I calculate the sample concentration?

The sample concentration is calculated based on four parameter fit logistic model. Usually Plate leader is equipped by such program, and we recommend to use the program. If you do not have such program, you can use a sample Excel program. Please be sure that the program is presented for free charge and Beacle Inc. takes any responsibility to the problems related with the program usage and the results. Get Free Program.

Experimental examples

For experimantal example of each kit see each flyer

Manual and MSDS

HB Pre-S1 Antigen Quantitative ELISA kit, Rapid:
Manual(Pre-S1 kit) MSDS(PEG) MSDS(TMB)
HB Pre-S2 Antigen Quantitative ELISA kit, Rapid:
Manual(Pre-S2 kit) MSDS(PEG) MSDS(TMB)
HB S Antigen Quantitative ELISA kit, Rapid-II:
Manual(S kit) MSDS(TMB)

for Antibody ELISA kits in prepareation