HBV-related antigens
Introduction
HBsAg (also called Bionanocapsule) is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials, and developed the production process of various HBsAgs . In this page, we introduce these HBsAg-related products.
General flyer for HBV-related antigens
Products listed in this page can be ordered directly to us through order form.
Properties of antigens
Our HBs antigens are all recombinant antigen produced in yeast or E.coli. Therefore, antigens shows stable quality, and does not cause HBV infections.
Types of antigens
1. HBsAg L-protein
Particulate HBsAg composed of L protein that has all three (S、Pre-S1、Pre-S2) domains, also called L antigen or large S antigen. The product is produced in recombinant yeast. Because all the three domains can produce antibodies and are recognized by each domain-specific antibodies, the product is ideal as an universal antigens. In addition, the antigen can be used as a tool to investigate HBV infection. Four products with different genotypes are available; genotype A, B, C and D. For detail see L-antigen datasheet.
2. HBsAg L-protein ST
Particulate HBsAg composed of L protein that has all three (S、Pre-S1、Pre-S2) domains, also called L antigen or large S antigen. The product is produced in recombinant yeast. Amino acids of S domain is replaced by naturally occurring escape mutants so that this antigen does not work as normal S-antigen; it is not recognized by ordinary anti-HBsAg (S) antibody and does not work as antigen to produce normal anti-HBsAg (S) antibody. Because other domains are normal and recognized as each specific antibodies, it can be used as a mimetic of HBV escape mutant or an antigen specific for Pre-S1 and Pre-S2 domains. The antigen may be useful to investigate HBV infection mechanism since it can escape from normal anti-S antibody. The genotype is C. For detail see L-antigen ST type datasheet.
3. HBsAg M-protein
Particulate HBsAg composed of M-proteins that have S and Pre-S2 domains, also called M antigen. The product is produced in recombinant yeast. M antigen is found in infected subjects as VLP. In addition, the antigen can be used as a tool to investigate HBV infection. The genotype is C. For detail see M-antigen datasheet. The product is supplied by order-based production (leading time about 1 month). Please ask us from inquiry form.
4. HBsAg XT
HBsAg showing high S-antigen activity. The antigen shows 3- to 5-fold higher S-antigen activity as compared with a typical HBV infected serum used as the HBsAg standard.The antigen can be used as the standard for HBsAg determination, antigens for anti-HBsAg antibody detection system, and others. The genotype is C. For detail see HBsAg-XT datasheet.
5. HBsAg S-protein
HBs S antigen (so called HBsAg) produced in yeast. Two subtypes, adw and adr are available. The antigen can be used as the standard for ELISA or western, for antibody detection ELISA, and others. For detail see HBsAg-S datasheet. The product is supplied by order-based production (leading time about 1 month). Please ask us from inquiry form.
6. Purified HBsAg from human serum
Particulate HBsAg purified from human serum. Four subtypes, adw, ayw, adr and ayr are available. The products are heat-inactivated and minimized infection risk. The antigen can be used as the standard for ELISA or western, for antibody detection ELISA, and others. For detail see human HBsAg-S datasheet. The product is supplied by order-based production by our contract organization (leading time about 1 month). Please ask us from inquiry form.
7. HBcAg
HBV Core antigen (HBcAg) is the particle antigen composed of full length HBV Core protein. The product is produced in E.coli. HBcAg datasheet.
8. Pre-S1 peptide
Pre-S1 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For each products, four peptides of different genotypes are available; genotype A, B, C and D. For detail please check Pre-S1 peptides datasheet.
9. Pre-S2 peptide
Pre-S2 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For each products, four peptides of different genotypes are available; genotype A, B, C and D. For detail please check Pre-S2 peptides datasheet.
10.Others
Other HBV antigens, and labeled products of above antigens are available on request. Please contact us from here.
Products and Price
Particulate Antigens| Product # | Product name | Content | Price |
|---|---|---|---|
| BCL-AGA-01 | HBsAg L-protein(gtA)（Recombinant） | 100μg | inquiry |
| BCL-AGB-01 | HBsAg L-protein(gtB)（Recombinant） | 100μg | inquiry |
| BCL-AGC-01* | HBsAg L-protein(gtC)（Recombinant） | 100μg | ￥100,000 |
| BCL-AGD-01 | HBsAg L-protein(gtD)（Recombinant） | 100μg | inquiry |
| BCL-AGS-02 | HBsAg L-protein ST（Recombinant） | 100μg | ￥100,000 |
| BCL-AGM-01 | HBsAg M-protein（Recombinant） | 100μg | inquiry |
| BCL-AGSC-01 | HBsAg S-protein（adr Recombinant） | 100μg | inquiry |
| BCL-AGSA-01 | HBsAg S-protein（adw Recombinant） | 100μg | inquiry |
| BCL-AGX-02** | HBsAg-XT（Recombinant） | 100μg | ￥100,000 |
| BCL-HSYW-01 | purified HBsAg from human serum, ay | 100μg | inquiry |
| BCL-HSDW-01 | purified HBsAg from human serum, ad | 100μg | inquiry |
| BCL-HSDR | purified HBsAg from human serum,adr | 100μg | discontinued |
| BCL-HSYR | purified HBsAg from human serum,ayr | 100μg | discontinued |
| BCL-HBC-01 | Hepatitis B virus Core antigen(recombinant) | 100μg | ￥100,000 |
#:Product noted “inquiry” requires 3 to 4 weeks for shipping.
All products have larger scale economical package. Please ask us from inquiry form.
Peptide Antigen Products and Price
| Product # | Product name | Content | Price |
|---|---|---|---|
| BCL-AGS1A-01 | HBsAg,Pre-S1 peptide(gtA)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS1B-01 | HBsAg,Pre-S1 peptide(gtB)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS1C-02 | HBsAg,Pre-S1 peptide(gtC)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS1D-01 | HBsAg,Pre-S1 peptide(gtD)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS1A-21 | HBsAg,Pre-S1 peptide(gtA)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS1B-21 | HBsAg,Pre-S1 peptide(gtB)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS1C-22 | HBsAg,Pre-S1 peptide(gtC)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS1D-21 | HBsAg,Pre-S1 peptide(gtD)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS2A-01 | HBsAg,Pre-S2 peptide(gtA)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS2B-01 | HBsAg,Pre-S2 peptide(gtB)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS2C-02 | HBsAg,Pre-S2 peptide(gtC)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS2D-01 | HBsAg,Pre-S2 peptide(gtD)（Recombinant,no Tag） | 100μg | ￥100,000 |
| BCL-AGS2A-21 | HBsAg,Pre-S2 peptide(gtA)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS2B-21 | HBsAg,Pre-S2 peptide(gtB)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS2C-22 | HBsAg,Pre-S2 peptide(gtC)（Recombinant,Tagged） | 100μg | ￥75,000 |
| BCL-AGS2D-21 | HBsAg,Pre-S2 peptide(gtD)（Recombinant,Tagged） | 100μg | ￥75,000 |
Q&A
Q: How long does the antigen stable？
Under the condition recommended by product, they are stable for more than one year.
Examples
An experimental examples using HBsAg-XT is shown here. Other examples using these products can be also seen in each datasheet.