TMB Solution
Product outline
TMB Solution is the substrate solution of HRP. Two types of products are available; One reagent type for ready to use and Two reagnet type which needs mix of equal volume of two solutions. Either type produces similar signal intensity to that observed with well-known widely used high end product.
Principle
Enzyme horse radish peroxidase (HRP) is the most popular enzyme for antibody labeling. HRP cause chromogenic reaction to TMB by the oxidative agent H2O2, which shows blue color at maximal absorption of 370nm and 652nm. By stop solution with H2SO4, the color changes to yellow at maximal absorption of 450nm.
Product feature
1. Good lineality against enzyme volume
2. Equal signal intensity as widely used high signal TMB solution
3. High cost performance
4. Good storage stability (1 year at 4°C)
Product lineup
The variation and price of TMB Solution is listed below.
| Product # | Product name | content | Price |
|---|---|---|---|
| BCL-TMB-01 | TMB solution (1 reagent type) | 250 mL | Temporary suspension |
| BCL-TMB-21 | TMB solution (2 reagent type) | 250 mL X 2 | ￥19,500 |
| BCL-TMS-01 | TMB Stop Solution | 100mL | ￥11,000 |
Q&A
Q: How long can the product be stored
Over 6 months at 4°C.
Experimental examples
Please check the backside of the flyer.
Manual and SDS
TMB solutionTMB Stop solution
Manual SDS(MA)