Blocking Solutions

Product outline

Because the selection of blocking agent is very important to get best result, we have developed 4 types of Blocking Solutions for each field of Western blot and ELISA. Blocking is performed to minimize the non-specific binding of antigens or antibodies. The chemical property of each antigen, antibodies and the material where reaction occurs all of which are the cause of non-specific binding, blocking agent has to be selected to get maximum results. The blocking solutions for ELISA are desinged to long-term preservation of pre-coated plate.

flyer for Blocking solutions

Selection of Blocking agents

Blocking Solutions have following main ingredients and characteristics ;
c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
h-Block:Casein-based. Good for many applications.
k-Block:degnatured casein-based. Shows stable results in many applications.
b-Block:BSA-based. Good for many applications.
Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution

Selection of Blocking agents
A general rule is described. We strongly recommend to select the best one by screening using Trila Set.
For Western Bot Many reserachers seems to use Skim milk. Skim milk is not bad choice, but it very often gives you weak signal due to its strong blocking action. Below is the recommendated use of our bloking solutions.
①To get higher signal（accept some background increase）：k-Block, c-Block
②To reduce background（accept some decrease of signal）：b-Block, h-Block
③To use as a standard blocking：h-Block, b-Block
④To detect phospho-protein：c-Block, b-Block
For ELISA　Below shows choice of blocking solutions depending on the ELISA system you use. All the solutions for ELISA are desinged for long term preservation, and dryed precoated plate can be stored for a few months after blocking at 4℃.
Antigen-detecting ELISA：
　Direct method (detect antigen adsorbed on plate by detecting antibody)：c-Block, b-Block, k-Block
　Antibody-sandwich method (detect antigen by captured antibody by detect antibody）：k-Block, b-Block, h-Block
Antibody-detecting ELISA：
　Direct method (detect antibody captured by pre-coated antigen)：c-Block, b-Block, k-Block
　Antigen-sandwich method (detect antibody by captured antigen by detect antigen）:k-Block, b-Block, h-Block　

Product feature

1. Ready-to-Use
2. Optimized blocking solution for WB and ELISA
3. For WB, stronger signal can be obtained by using Skim milk*
4. For ELISA, pre-coated plate can be stored for months

*： based on experiments in our laboratory

Product lineup

WB Blocking Solutions

Blocking solution Trial set contains all the four solutions, and good for a screening to select the best Blocking solution.

ELISA Blocking solution

Blocking solution Trial set contains all the four solutions, and good for a screening to select the best Blocking solution.
ELISA blocking solutions is good for storage of pre-coated plate for months. 　

 Blocking solution

Q&A

Q:　How long can the solutions stored?

At least 1 year at 4°C

Q:　Can we use them after dilution?

All solutions are optimized and we do not recommend dilution.

Experimental examples

Please check the backside of the flyer.

Manual and MSDS

Manual and MSDS download form here

Blocking Solution manual

MSDS not prepared since no regulated material is used for these products.