Antibody-binding particles for Immunoassays

Antibody-binding particle for Immunoassays

Introduction

We produce antibody-binding BNC. The BNC is a nano-sized (55 nm) particle consisted of about 100 proteins displaying antibody binding sites. The particle labelled with molecules for signal generation (such as HRP and Fluorescent dyes) is called MAD (Multi Antibody Detection) reagent. The representitive of MAD reagent is HRP-labelled one which is labelled about 100 HRP molecules per particle. MAD reagent binds to antibodies at high affinity, and be used for various immunoassys as high sensitivity.
Further studies are on going to improve the MAD technology.

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Please click HERE to download the flyer

Principle

02Antibody-binding BNC has 200 to 400 antibody-binding sites per particle depending on the type. The antibody binding site is originated from the biding domain of antibody-binding protein, protein A and/or protein G. Because antibodies bind to these binding sites through their Fc region, the antigen binding ability of them is little affected by the binding. When BNC and IgG are mixed, they form a complex which displays many Fab domains outside as shown in the right figure. The MAD reagent is antibody-binding BNC labeled with molecules for signal generation, such as HRP and fluorescent dye. For example, HRP-labelled MAD reagent is composed of antibody-binding BNC which is labeled about 100 HRP molecules per particle, providing highly sensitive antibody detection
As seen in the below figures, in ordinary method, antibodies bound to antigen is detected by 2nd antibody, such as HRP-labelled antibody. In MAD method, the antibodies are detected by MAD reagent. Because MAD reagent has much more antibody-binding sites (more than 100-fold) and is labelled with much more HRP (at least 50-fold more) than 2nd antibody, MAD reagent works more sensitive antibody sensor than 2nd antibodies. Furthermore, MAD reagent provide one-step antigen detector by using the MAD-antibody complex.

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Product lineup

1. BNC-ZZ (Bionanocapsule-ZZ)
The product, BNC-ZZ, is particle composed of proteins displaying 2 Z-domain (antibody-binding domain from protein A). BNC-ZZ has week affinity to some antibodies such as mouse IgG1 and Goat IgG. For the usage and other properties of BNC-ZZ refer to Data Sheet

2. HRP-MAD reagent
The product is BNC-ZZ labeled with 100 HRP molecules per particle. Good as the substitute of 2nd antibody, in Western blot, antibody-detecting ELISA , antigen-detecting ELISA using direct method. Kit products using HRP MAD reagent is also available. If you want to use the MAD reagent for your purpose, Please make contact us. We will provide you suitable advice or protocol.
Easy-WESTERN-II Flyer and Manual.
Easy ELISA constructor Flyer and Manual.

3. ALP-MAD reagent
The product is BNC-ZZ labeled with 20 ALP (alkaline phosphatase) molecules per particle. Good as the substitute of 2nd antibody, in Western blot, antibody-detecting ELISA , antigen-detecting ELISA using direct method. If you want to use the ALP-MAD reagent, please make contact us. We will provide you suitable advice.

4. Fluorescent MAD reagent
The product is BNC-ZZ labeled with Cy dyes. This product can be used for simultaneous multi-protein detecting western blot with multi-color (IRODORI) which is developed by Prof. Kuroda and colleagues of Osaka Univ. The probe can be also used for immunolabelling of multi-antigens in flow cytometry, immunostaining, or fluorescent immunoassays. The fluorescent MAD reagent is produced by order, so Please contact us if you wish to use the probe.

Antibody-binding BNC and MAD can be uses in many ways. The representative ways are summarized in below table.

Antibody binding BNC alignment of immobilizing antibodies in ELISA (plate-bound BNC)
Purification or elimination of IgG(resin-bound BNC)
Detection of IgGs in immuno-chromatograpy (membrane-bound BNC)
Highly sensitive IgG detection (using QCM)
HRP-labelled MAD reagent* Western blot Sensitivity increase (much increase by simultaneous use with 2nd antibody)
Simple reprobing
Simultaneous detection of multi proteins
Rapid detection using one-step method
Antibody detecting ELISA Sensitivity increase (much increase by simultaneous use with 2nd antibody)
Simultaneous detection of multi antigens
Rapid detection using one-step method
Antigen detecting ELISA by direct method Sensitivity increase (much increase by simultaneous use with 2nd antibody)
Fluorescent MAD reagent Western blot Simultaneous detection of several proteins with multicolor (IRODORI)
Flow cytometry Simultaneous immunolabeling of multiple antigens
Immunohistochemistry Simultaneous immunolabeling of multiple antigens

*:ALP-labelled MAD reagent can also be used.
We can provide protocol or references for the above listed use

Price

Product # Product name Product outline Content Price
BCL-DC-002 Bionanocapsule-ZZ(BNC-ZZ) BNC displaying antibody binding site originated from protein A 100μg ¥80,000
BCL-MA-001 HRP-labelled MAD Reagent HRP-labeledd BNC-ZZ 100μg ¥150,000
BCL-MP-001 ALP-labelled MAD Reagent ALP-labeledd BNC-ZZ(production by order) 100μg ¥150,000
BCL-MC-001 to -004 Fluorescent dye-labelled MAD Reagent Cy2, Cy3, Cy5, or Cy7-labelled BNC-ZZ(production by order) 50μg ¥80,000 each

labeling with other molecule is possible. Please contact us.

Q&A

Q: How long is the preservation time?

More then 1 year as long as the product is preserved in the recommended condition.

Example

For IRODORI, refer to reference and IRODORI protocol.
For the immunohistochemistry and flow cytometry using fluorescent MAD, refer to reference.