HBsAg S, Pre-S1, Pre-S2 Qualitative Kits

HBsAg S, Pre-S1, Pre-S2 Qualitative Kits

Product outline

Owing to deep research experience in HBV envelop, Beacle launches quantitative ELISA kits targeted to varous HBsAg domains. Three types of HBsAg is known; named S-, M- and L-antigen. L-antigen is consisted of S, Pre-S2 and Pre-S1 domains, M-antigen of S and Pre-S2 domains, and S-antigen of S domain alone. These domains play important role in HBV infection. Pre-S1 domain is known to be the key element in recognizing hepatic cells, Pre-S2 and S is important in cellular invasion fo HBV. The serum level of S-antigen (or S-domain) is well studies as a tool to daignose the HBV infection, but almost no study was conducted to examine the serum level of Pre-S1 and Pre-S2. Beacle, by marketing ELISA kit to detect these domains, wishes to contribute studies of HBV infection.
Products listed in this page can be ordered directly to us through oder form, or can be odered through our distributors B-Bridge International (USA, Europe), Takara-Bio, Cosmobio, or Funakoshi.

Products properties

Pre-S1 quantitative ELISA kit, Rapid
Only one product in the world that can detect Pre-S1 domains
・High sensitivity:detection limit is 0.1 nUnit/ml
・Possible to determine Pre-S1 antivity in human serum or plasma
・High specificity: Sandwich system using two types of anti-Pre-S1 antibodies
For detail see leaflet

Pre-S2 quantitative ELISA kit, Rapid
Only one product in the world that can detect Pre-S2 domains
・High sensitivity:detection limit is 0.1 nUnit/ml
・High specificity:Sandwich system using two types of anti-Pre-S2 antibodies
For detail see leaflet

S-antigen quantitative kit, Rapid-II
・High sensitivity:detection limit is 0.05 nUnit/ml (1 nUnit is roguhly equal to 0.5 to 1 IU of WHO HBsAg standard
・High specificity:Sandwich system using two types of anti-S antibodies
For detail see leaflet

(Composition)
Kits are equipped with all the necessary components shown below.
・Detection IgG
・Dilution solution for Detection IgG
・Dilution buffer for antigens
・Washing buffer
・Chromogenic Reagent
・Stop Solution
・Pre-coated Microplate
・Working manual
For detail please check the working manual for each product
For easier handling, choromogenic reagent and stop solution have been recently changed. Make sure the change of protocol.

Product lineup and Price

Product # Product name Quantity Price
BCL-S1HP-01 HB Pre-S1 Antigen Quantitative ELISA Kit 1 Kit (96 assay) ¥80,000
BCL-S2HP-01 HB Pre-S2 Antigen Quantitative ELISA Kit 1 Kit (96 assay) ¥80,000
BCL-SHP-21 HB S Antigen Quantitative ELISA Kit 1 Kit (96 assay) ¥60,000

Related products

Product # Product name Quantity Price
BCL-AGS-02 Recombinant HBsAg L-protein ST, Pre-S1 and Pre-S2 100μg ¥80,000
BCL-AG-001 Recombinant HBsAg L-protein 100μg ¥80,000
BCL-AB-002 Anti Pre-S1 antibody, mouse-mono-2 100μg ¥100,000
BCL-AGX-002 HBsAg-XT(Recombinant HBsAg with high antigenic activity) 100μg ¥80,000

For other antigens and antibodies, refer to antigen and antibody page.

Q&A

For HB Pre-S1 Antigen Quantitative ELISA Kit

Q: How long the kit can preserve?

All the component of the kit is stable for at least 6 months at 4℃.

Q: How long does it take for an assay

The approximate time required for an assay is shown below.
1) Reaction with capture IgG and detection IgG: 60 min→5)Chromogenic reacton: 30 min

Q: What is the definition of PreS-1 activity?

There is no common and standard definition of Pre-S1 antigen activity. In the kit the activity is expressed by our unit system, where 1 nUnit equal to the Pre-S1 activity expressed by 1ng of our standard antigen.

Q: What is the standrd antigen?

The standard antigen are the recombinant HBsAgs produced from yeast. These antigens gives reproduceble antigen activity, and no risk for HBV infection.

Q: How can I calculate the sample concentration?

The sample concentration is calculated based on four parameter fit logistic model. Usually Plate leader is equipped by such program, and we recommend to use the program. If you do not have such program, you can use a sample Excel program. Please be sure that the program is presented for free charge and Beacle Inc. takes any responsibility to the problems reated with the program usage and the results. Get Free Program.

Experimental examples

For experimantal example of each kit see below files
HB Pre-S1 Antigen Quantitative ELISA Kit example(in preparation)
HB Pre-S2 Antigen Quantitative ELISA Kit example(in preparation)
HB S Antigen Quantitative ELISA Kit example(in preparation)

Manual and MSDS

HB Pre-S1 Antigen Quantitative ELISA kit, Rapid:
Manual(Pre-S1 kit) MSDS(PEG) MSDS(TMB)

HB Pre-S2 Antigen Quantitative ELISA kit, Rapid:
Manual(Pre-S2 kit) MSDS(PEG) MSDS(TMB)

HB S Antigen Quantitative ELISA kit, Rapid-II:
Manual(S kit) MSDS(TMB)