HBsAg antigens

Various HBsAg antigens and Antibodies

Introduction

HBsAg (also called Bionanocapsule) is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle(diameter 50 to 60nm)in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials, and developed the production process of various HBsAgs . In this page, we introduce these HBsAg-related products.

Products listed in this page can be ordered directly to us through order form.

Properties of antigens

Our HBs antigens are all recombinant antigen produced in yeast or E.coli. Therefore, antigens shows stable quality, and does not cause HBV infections.

Types of antigens

1. HBsAg L-protein
Particulate HBsAg having all three (S、Pre-S1、Pre-S2) domains. Because all the three domains can be recognized by each domain-specific antibodies, the product is ideal as a universal antigens. In addition, the antigen can be used as a tool to investigate HBV infection. Four products with different genotypes are available; genotype A, B, C and D.  For detail see L-antigen datasheet.

2. HBsAg L-protein ST
HBsAg having all three (S、Pre-S1、Pre-S2)domains. The S domain is modified so that this antigen does not work as S-antigen; it is not recognized by ordinary anti-HBsAg (S) antibody and does not work as antigen to produce anti-HBsAg (S) antibody. Because other domains are recognized as each specific antibodies, it can be used as the specific antigen for Pre-S1 and Pre-S2. The antigen is useful to investigate HBV infection mechanism since it can escape from antibody even in HBV-infected blood. For detail see L-antigen ST type datasheet.

3. HBsAg XT
HBsAg showing high S-antigen activity. The antigen shows 3- to 5-fold higher S-antigen activity as compared with a typical HBV infected serum used as the HBsAg standard.The antigen can be used as the standard for HBsAg determination, antigens for anti-HBsAg antibody detection system, and others. For detail see HBsAg-XT datasheet.

4. HBsAg S-protein
HBs S antigen (so called HBsAg) produced in yeast. The antigen can be used as the standard for ELISA or western, for antibody detection ELISA, and others. The product is supplied by order-based production (leading time about 1 month). Please ask us from inquiry form.

5. HBcAg
Core antigen of Hepatitis B (HBcAg) is the particle antigen composed of HBV Core protein produced in E.coli.  Pre-S2 peptides datasheet.

6. Pre-S1 peptide
Pre-S1 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For each products, four peptides of  different genotypes are available; genotype A, B, C and D.  For detail please check Pre-S1 peptides datasheet.

7. Pre-S2 peptide
Pre-S2 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For each products, four peptides of  different genotypes are available; genotype A, B, C and D.  For detail please check Pre-S2 peptides datasheet.

8.Other HBs antigens
Various HBsAg antigens, such as S-antigen, M-antigen, and L-antigen of different genotypes, and others antigens are also available on request. Please contact to us from here.

Particulate Antigen Products and Price

Product #Product nameContent   Price
BCL-AG-01HBsAg L-protein(gtC)(Recombinant)100μg¥85,000
BCL-AGS-02HBsAg L-protein ST(Recombinant)100μg¥85,000
BCL-AGSC-01HBsAg S-protein(Recombinant)**
BCL-AGX-02**HBsAg-XT(Recombinant)100μg¥85,000
BCL-HBC-01Hepatitis B virus Core antigen(recombinant, low100μg¥85,000
BCL-HAD-01Purified HBsAg,subtype,adw-heat inactivated,preserved and filtered100μg¥85,000
BCL-HAY-01Purified HBsAg,subtype,ayw-heat inactivated,preserved and filtered100μg¥85,000

Peptide Antigen Products and Price

Product #Product nameContent   Price
BCL-AGS1A-01HBsAg,Pre-S1 peptide(gtA)(Recombinant,no Tag)100μg¥85,000
BCL-AGS1B-01HBsAg,Pre-S1 peptide(gtB)(Recombinant,no Tag)100μg¥85,000
BCL-AGS1-02HBsAg,Pre-S1 peptide(gtC)(Recombinant,no Tag)100μg¥85,000
BCL-AGS1D-01HBsAg,Pre-S1 peptide(gtD)(Recombinant,no Tag)100μg¥85,000
BCL-AGS1A-21HBsAg,Pre-S1 peptide(gtA)(Recombinant,Tagged)100μg¥65,000
BCL-AGS1B-21HBsAg,Pre-S1 peptide(gtB)(Recombinant,Tagged)100μg¥65,000
BCL-AGS1-21HBsAg,Pre-S1 peptide(gtC)(Recombinant,Tagged)100μg¥65,000
BCL-AGS1D-21HBsAg,Pre-S1 peptide(gtD)(Recombinant,Tagged)100μg¥65,000
BCL-AGS2A-01HBsAg,Pre-S2 peptide(gtA)(Recombinant,no Tag)100μg¥85,000
BCL-AGS2B-01HBsAg,Pre-S2 peptide(gtB)(Recombinant,no Tag)100μg¥85,000
BCL-AGS2-01HBsAg,Pre-S2 peptide(gtC)(Recombinant,no Tag)100μg¥85,000
BCL-AGS2D-01HBsAg,Pre-S2 peptide(gtD)(Recombinant,no Tag)100μg¥85,000
BCL-AGS2A-21HBsAg,Pre-S2 peptide(gtA)(Recombinant,Tagged)100μg¥65,000
BCL-AGS2B-21HBsAg,Pre-S2 peptide(gtB)(Recombinant,Tagged)100μg¥65,000
BCL-AGS2-21HBsAg,Pre-S2 peptide(gtC)(Recombinant,Tagged)100μg¥65,000
BCL-AGS2D-21HBsAg,Pre-S2 peptide(gtD)(Recombinant,Tagged)100μg¥65,000

Q&A

Q: How long does the antigen stable?

Under the condition recommended by product, they are stable for more than one year.

Examples

An experimental examples using HBsAg-XT is shown here. Other examples using these products can be also seen in each datasheet.

Antibody-detecting ELISA constraction using HBsAg-XT