Various HBsAg antigens and Antibodies

Various HBsAg antigens and Antibodies

Introduction

HBsAg is the surface antigen of Hepatiis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle(diameter 50 to 60nm)in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (haptic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials, and developed the production process of various HBsAgs and Bionanocapsule (a kind of form of HBsAg) for DDS purpose. We now supply these HBsAg-related products.
Products listed in this page can be ordered directly to us through oder form, or from our distributors, B-Bridge International (US and Europe, CA) or Funakoshi or Cosmo-Bio (worldwide, JP).

Properties of antigens and antibodies

Our HBs antigens are all recombinant antigen produced in yeast or E.coli. Therefore, antigens shows stable quality, and does not cause HBV infections. We also supply HBs-related antibodies, good for western and ELISA.

Types of antigens and antibodies

We sell antigens listed below.

1. HBsAg L-protein
HBsAg having all three (S、Pre-S1、Pre-S2)domains. Because all the three domains can be recognized by each domain-specifec antibodies, teh product is ideal as a universal antigens. In addtion, the antigen can be used as a tool to investigate HBV infection. For detail see L-antigen datasheet.

2. HBsAg L-protein ST
HBsAg having all three (S、Pre-S1、Pre-S2)domains. The S domain is modified so that this antigen does not work as S-antigen; it is not recognized by ordinary anti-HBsAg(S) antibody and does not work as antigen to produce anti-HBsAg(S) antibody. Because other domains are recognized as each specific antibodies, it can be used as the specific antigen for Pre-S1 and Pre-S2. The antigen is useful to investigate HBV infection mechanism since it can escape from antibody even in HBV-infected blood. For detail see L-antigen ST type datasheet.

3. HBsAg XT
HBsAg showing high S-antigen activity. The antigen shows 3- to 5-fold higher S-antigen activity as compared with a typical HBV infected serum used as the HBsAg standard.The antigen can be used as the standard for HBsAg determination, antigens for anti-HBsAg antibody detection system, and others. For detail see HBsAg-XT datasheet.

4. HBsAg S-protein
HBs S antigen (so called HBsAg) produced in yeast. The antigen can be used as the standard for ELISA or western, for antibody detection ELISA, and others. The product is supplyed by order-based production (leading time about 1 month). Please ask us from inquiry form.

5. Pre-S1 peptide
Pre-S1 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For detail please check Pre-S1 peptides datasheet.

6. Pre-S2 peptide
Pre-S2 peptide produced in E.coli. There are two types of products, one is tagged peptide and the other is no tagged peptide. In general, the former product is good for immunoassay use, and the latter for standard use. For detail please check Pre-S2 peptides datasheet.

7. Anti-HBs S antibody(polyclonal)
Polyclonal antibody raised against HBs S-protein (so called HBsAg) in rabbit. The product can be used for western, and ELISA. For the detail please check HBsAg polyclonal antibody datasheet. “HBs S-protein detecting ELISA kit” is also available. Click here for “ELISA kit“.

8. Anti-HBs Pre-S1 antibody(polyclonal)
Polyclonal antibody raised against HBs Pre-S1 peptide in rabbit. The product can be used for western, and ELISA. For the detail please check Pre-S1 polyclonal antibody datasheet. “Pre-S1 detecting ELISA kit” is also available. Click here for “ELISA kit“.

9. Anti-HBs Pre-S1 antibodies (monoclonal)
Two types of anti-Pre-S1 antibodies are available. One can be for Western blotting. A sandwich ELISA system can be established by using these two antibodies or with above polyclonal antibody. For detail see Pre-S1 mono 1 datasheet , and Pre-S1 mono 2 datasheet. “Pre-S1 detecting ELISA kit” is also available. Click here for “ELISA kit“.

10. Anti-HBs Pre-S2 antibody(polyclonal)
Polyclonal antibody raised against HBs Pre-S2 peptide in rabbit. The product can be used for western, and ELISA. For the detail please check Pre-S2 polyclonal antibody datasheet. “Pre-S2 detecting ELISA kit” is also available. Click here for “ELISA kit“.

11. Anti-HBs Pre-S2 antibody(monoclonal)
Mouse monoclonal antibody against Pre-S2reagion. Antigen is HBsAg-L protein. The product is useful for western blot and ELISA. For detail see Pre-S2 monoclonal antibody datasheet. “Pre-S2 detecting ELISA kit” is also available. Click here for “ELISA kit“.

12.Other HBs antigens
Various HBsAg antigens, such as S-antigen, M-antigen, and others are available on request. Please contact to us from here.

Products and Price

Product # Product name Content Price
BCL-AG-001 HBsAg L-protein (Recombinant) 100μg ¥80,000
BCL-AGS-02 HBsAg L-protein ST (Recombinant) 100μg ¥80,000
BCL-AGSC-01 HBsAg S-protein (Recombinant) Produced on request Contact us
BCL-AGX-02 HBsAg-XT (Recombinant) 100μg ¥80,000
BCL-AGS1-01 HBsAg,Pre-S1 peptide(Recombinant,no Tag) 100μg ¥80,000
BCL-AGS1-21 HBsAg,Pre-S1 peptide(Recombinant,Tagged) 100μg ¥60,000
BCL-AGS2-01 HBsAg,Pre-S2 peptide(Recombinant,no Tag) 100μg ¥80,000
BCL-AGS2-21 HBsAg,Pre-S2 peptide(Recombinant,Tagged) 100μg ¥60,000
BCL-ABPS-01 Anti-HBs, S-protein, Rabbit-Poly 100μg ¥30,000
BCL-AB-001 Anti-HBs Pre-S1, mono 1 100μg ¥30,000
BCL-AB-002 Anti-HBs Pre-S1, mono 2 100μg ¥100,000
BCL-ABP1-01 Anti-HBs Pre-S1, Rabbit-Poly 100μg ¥30,000
BCL-ABP2-01 Anti-HBs Pre-S2, Rabbit-Poly 100μg ¥30,000
BCL-ABM2-01 Anti-HBs Pre-S2, Mouse-Mono 100μg ¥60,000
BCL-DC-002 Bionanocapsule-ZZ 100μg ¥80,000

Q&A

Q: How long does the antigen stable?

Under the codition recommended by product, they are stable for more than one year.

Examples

An experimental examples using HBsAg-XT is shown here. Other examples using these products can be also seen in each datasheet.

Antibody-detecting ELISA constraction using HBsAg-XT